Melatonin attenuates lung injury in a hind limb ischemia–reperfusion rat model
Hamed Takhtfooladia, Mohammad Takhtfooladib,, , Fariborz Moayerc, Sayed Mobarakehd
a Faculty of Veterinary Science, Karaj Branch, Islamic Azad University, Karaj, Iran
b Department of Surgery, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
c Department of Pathobiology, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
d Faculty of Medical Sciences, Shahid Sadughi University, Yazd, Iran

This study evaluated the protective antioxidant effect of melatonin on lung injury as a remote organ after skeletal muscle ischemia–reperfusion in rats.


Thirty male Wistar rats were allocated randomly into three experimental groups: operated with no ischemia (Sham) group, ischemia–reperfusion group and ischemia–reperfusion + melatonin group. Hind limb ischemia was induced by clamping the femoral artery. After 2 h ischemia, the clamp was removed and the animal underwent 24 h reperfusion. Rats in the ischemia–reperfusion + melatonin group received melatonin (10 mg/kg i.v.), immediately before the clamp was removed. At the end of the trial, animals were euthanized and the lungs were removed for water content determination, histopathological and biochemical studies.


In the ischemia–reperfusion + melatonin group, tissues showed less intense histological abnormalities such as neutrophilic infiltration, intra-alveolar hemorrhage and edema compared with the ischemia–reperfusion group. Histopathologically, there was a significant difference (P < 0.05) between the two groups. The lung water content in the ischemia–reperfusion + melatonin group was significantly lower than the ischemia–reperfusion group (P < 0.05). Lung tissue myeloperoxidase (MPO) activity and nitric oxide (NO) level were significantly (P < 0.05) increased by ischemia–reperfusion. The increase in these parameters was reduced by melatonin.

Comparing the ischemia–reperfusion + melatonin group with the sham group, no significant increase in all analyzed aspects of the research was observed.


These findings suggest that melatonin has preventive effects in lung tissue injury after transient femoral artery occlusion.

Melatonin, Ischemia–reperfusion, Lung remote injury, Histopathology, Myeloperoxidase, Nitric oxide
Journal: Melatonin attenuates lung injury in a hind limb ischemia–reperfusion rat model

This article belongs to the Journal: Pulmonology


  • Impact Factor: 1.731(2017)
  • 5-years Impact Factor: 1,334
  • SCImago Journal Rank (SJR):0,342
  • Source Normalized Impact per Paper (SNIP):549